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. 2001 Apr 30;153(3):503–516. doi: 10.1083/jcb.153.3.503

Figure 7.

Figure 7

(A–D) Time-lapse observations of GFP-K18 are made in a live PtK2 cell after treatment with 20 μM cytochalasin B for 30 min. Cell margins are retracted, and the overall morphology is arborized as seen by phase–contrast (A). Keratin squiggles (B) are present in the arborized regions of the same cell (arrow). (C and D) Two motile keratin squiggles are indicated by arrows. (E–H) PtK2 cells were treated with 6 μM nocodazole for 5 h to disassemble MTs. GFP-K18 tonofibrils show extensive wave-like movements. Elapsed time (min:s) is indicated at the lower left of each confocal image. Bars, 5 μm.