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. 2001 Apr 30;153(3):585–598. doi: 10.1083/jcb.153.3.585

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Coimmunoprecipitation of CH-ILKBP and ILK with FLAG-PINCH. (A and B) FLAG-PINCH was immunoprecipitated from lysates of C2C12 cells expressing FLAG-PINCH. FLAG-PINCH immunoprecipitates (lane 3) or control precipitates (lane 4) were analyzed by Western blotting with anti-FLAG antibody M2 (A), anti-ILK antibody 65.1 (B), or anti–CH-ILKBP antibody 3B5 (C). Lanes 1 and 2 were loaded with lysates (7.5 μg protein/lane) of the FLAG-PINCH–expressing C2C12 cells and the control C2C12 cells, respectively. (D and E) Antipaxillin immunoprecipitates (lane 2) or control IgG precipitates (lane 3) were blotted with antipaxillin antibody (D) or anti–CH-ILKBP antibody 3B5 (E). (Lane 1) 10 μg lysates; (lane 4) 0.4 μg antipaxillin IgG.