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. 2001 Apr 30;153(3):585–598. doi: 10.1083/jcb.153.3.585

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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FA localization and cytoskeleton association of CH-ILKBP. (A–F) Subcellular localization of CH-ILKBP. Primary rat mesangial cells were stained with mouse anti–CH-ILKBP antibody 1D4 and rabbit anti-FAK antibodies (A and B) or anti–CH-ILKBP antibody 1D4 and phalloidin (C and D). We have also stained IEC18 rat intestinal epithelial cells, CHO cells, and mouse C2C12 cells with anti–CH-ILKBP antibodies and found that CH-ILKBP localizes to FA in these cells as well. Immunofluorescence images of IEC18 cells stained with mouse anti–CH-ILKBP antibody 1D4 (E) and rabbit anti–β-catenin antibody (F). (G–I) Association of CH-ILKBP with Triton X-100–insoluble cytoskeleton fractions. CH-ILKBP, FAK, and p44/42 ERK in the Triton X-100–soluble and –insoluble fractions were detected by Western blotting with anti–CH-ILKBP antibody 3B5 (G), anti-FAK antibody (H), and anti-p44/42 ERK antibody (New England Biolabs, Inc.) (I), respectively. Bar, 5 μm.