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. 2000 Dec 11;151(6):1295–1304. doi: 10.1083/jcb.151.6.1295

Figure 2.

Figure 2

Serial analysis of muscle differentiation–regulated gene expression. (a) Northern blot hybridizations were performed on total RNA extracted from C2C12 and C2-dnp53 cells incubated in DM for the indicated times, or maintained in GM. (b and c) Quantitative analyses, relative to GAPDH, of the gene expression levels observed in a were evaluated for MyHC and Rb in the indicated cells. (d) C2C12 and C2-dnp53 cells, incubated as in a, were analyzed by Western blotting for the listed proteins. The numbers represent quantitative data relative to Hsp70. (e) p53+/+ and p53−/− MSC were incubated as in a. p53−/− MSC were also transduced with the indicated gene by retroviral infection and incubated for 96 h in DM. Total cell lysates from each of these cell lines were analyzed by Western blotting for the listed proteins. (f) C2C12 and C2-dnp53 cells were incubated in GM, or in DM for 96 h, and maintained at 32°C. Northern blot hybridization was performed for the indicated genes. The numbers represent quantitative data relative to GAPDH.