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. 2000 Dec 11;151(6):1295–1304. doi: 10.1083/jcb.151.6.1295

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Rb promoter activity in muscle differentiation. (a) pRXP1-carrying C2C12 cells (C2-P1) were incubated in DM for different times. Time 0 indicates cells maintained in GM. Luciferase activity was normalized for number of nuclei. (b) Two different polyclonal populations of pRXP1-carrying C2C12 cells (C2-P1 Mix1 and C2-P1 Mix2) were incubated as in a. Columns report the luciferase activity of each cell line, relative to its own GM. (c) The region between −219 and −143 base-pairs upstream of the translation start site of the C2C12 endogenous Rb promoter was analyzed by in vivo footprinting in proliferating (P) and 48 h differentiated (D) cells; in vitro footprinting (V) was used as control. (Continuous arrows) Bands that are significantly protected; (dashed arrows) bands that are not protected; and (*) spurious bands. (d) Total RNA was extracted from C2C12 and C2-dnp53 cells maintained in GM or DM for 48 h, with or without Act D. Northern blottings were performed for the Rb and GAPDH genes. Histograms show quantitative analyses of the Rb gene expression levels relative to GAPDH.