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. 2000 Dec 11;151(6):1221–1234. doi: 10.1083/jcb.151.6.1221

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of CD34 and MyoD on satellite cells associated with isolated single muscle fibers. (A–C) A freshly isolated EDL single fiber immunostained for CD34 (A, red), MyoD (B, green), and counterstained with DAPI (C). (Arrow) A single CD34^+ve, MyoD^+ve satellite cell closely associated with an underlying myonucleus. (D–F) A single EDL fiber immunostained for CD34 (D, red), MyoD (E, green), and counterstained with DAPI (F) after 48 h in culture. Two CD34^+ve, MyoD^+ve satellite cells (arrows) are shown migrating off the parent fiber. The CD34 staining was punctate, in contrast to the continuous surface staining observed on quiescent cells. Bar, 30 μm. (G) RT-nested PCR analysis of CD34 isoform expression in isolated single fibers during activation in vitro. Each pair of gels shows the PCR products obtained from 12 individual fibers using primers for CD34 (left) and MyoD (right). Groups of fibers were taken immediately after isolation (0 h) and after 3 or 6 h of culture in the presence of horse serum. PCR products derived from the CD34^trunc and CD34^full alternately spliced transcripts are arrowed T (416 bp) and F (250 bp), respectively.