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. 2000 Dec 11;151(6):1155–1168. doi: 10.1083/jcb.151.6.1155

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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GFP-lamin A and B show intranuclear fluorescence in the majority of interphase PAM cells. (a–e) A live cell expressing GFP-lamin B shows nucleoplasmic (the lamin “veil”) and lamina fluorescence (a) with confocal optics. In b, the cell was photobleached, and after 240 min (c) the bleach zone could still be detected. The t _1/2 for both the nucleoplasm and lamina is similar (∼180 min, see text and Fig. 11). This veil can be seen throughout the nucleoplasm in a confocal through focus series (not shown). (d–f) Live PAM cells expressing GFP-lamin A have a more prominent nucleoplasmic veil. An optical section from an interphase cell expressing GFP-lamin A is shown just before (d), and just after (e) photobleaching. Note that the prominent bleach zone is retained across the entire nucleus, suggesting that this is not an early G1 cell (see text). In f, the same cell was extracted immediately after photobleaching with IF buffer and an image was captured. The veil fluorescence is greatly reduced, but lamina fluorescence appears unaltered. Bars, 10 μm.