Figure 2.

A series of confocal (a–d) and phase contrast (e–h) images of the same PAM cell expressing GFP-lamin B1 as it progresses from early telophase through nuclear assembly in daughter cells. The numbers in the lower right corner refer to the times after the metaphase to anaphase transition. (a and e) Increased fluorescence at the surface of the spindle pole side of the chromosome; (b and f) the same cell taken 2 min later, at which time lamin B1 appears to have completely enclosed the surface of the decondensing chromosomes in late telophase (note the overall reduction in cytoplasmic fluorescence); (c and g) 5 min later, showing that the vast majority of GFP-lamin B fluorescence is located at the surface the daughter cell nuclei, with almost no detectable cytoplasmic fluorescence; (d and h) the daughter cells ∼30 min after the completion of telophase. Note the apparent increase in size of the nucleus. Bar, 10 μm.