Figure 5.

PAM cells fixed and prepared for double-label indirect immunofluorescence using antibodies against lamins B or A/C and nucleoporins (Mb414, a–h), NUP153 (i and j), and LAP2β (k and l). A cell in telophase is shown stained with Mb414 (a) and lamin B antibody (b). Lamin B accumulates first at the spindle side of chromosomes before the 414-reactive nucleoporins have begun to accumulate. By the late stages of cytokinesis (early G1), both nucleoporins (c) and lamin B (d) are mainly located in the peripheral region of daughter cell nuclei. In late cytokinesis/early G1, Mb414 stains primarily the peripheral region of daughter cell nuclei (e) and in the same cell the majority of lamin A/C appears distributed throughout the cytoplasm. Later in G1, nucleoporins (g) and lamin A/C (h) are found primarily in the nucleus. Cells in anaphase/telophase show staining at the spindle pole side with NUP153 (i) before lamin B (j). Similarly, LAP2β (k) has completely accumulated around chromatin before lamin B (l). All fluorescence images are confocal. Bars, 10 μm.