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. 2000 Dec 11;151(6):1155–1168. doi: 10.1083/jcb.151.6.1155

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Confocal images of live PAM cells expressing GFP-lamin B1 before and after extraction with IF buffer. Cells were followed through nuclear assembly and at various time points, the culture medium was removed, and IF buffer was added. An image was collected ∼10 s after extraction. A live cell 20 min after lamin B enclosure (a) and immediately after extraction (b). At this time point, the GFP-lamin B1 remaining after extraction consists of discontinuous spots and lines around the nucleus (arrow). A live cell was also followed for 45 min after enclosure (c), and then extracted with IF buffer (d). In this case, the GFP-lamin B forms an apparently continuous rim around the nucleus after extraction. In both cases (a and b, c and d), there are cytoplasmic lamin foci that have been reported by immunofluorescence in early G1 cells (Goldman et al. 1992; Chaudhary and Courvalin 1993). Bar, 10 μm.