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. 2000 Dec 11;151(6):1257–1268. doi: 10.1083/jcb.151.6.1257

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Assembly of GFP–TIA-1⁺ SGs. COS cells were transfected with GFP–TIA-1 and cultured for 24 h; arsenite (0.5 mM) was then added to initiate the stress response. Cellular fluorescence was viewed and photographed using digital capture, pseudocolored using Meridian software, and compiled using Adobe Photoshop^®. The relative fluorescence intensity is indicated by the scale at the left in which green is the lowest and blue is the highest intensity. Times after addition of arsenite are indicated in each panel. A time lapse video of this process is available at http://www.jcb.org/cgi/content/full/151/6/1257/DC1.