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. 2000 Dec 11;151(6):1257–1268. doi: 10.1083/jcb.151.6.1257

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© 2000 The Rockefeller University Press

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Fluorescence recovery after photobleaching. Fluorescence microscopy of arsenite-treated (0.5 mM, 30 min) COS cells expressing GFP–TIA-1 (A and B) or GFP–PABP-I (C). Formaldehyde-fixed cells are viewed in A. Live cells are viewed in B and C. (left) Fluorescence intensity across a linear scan centered on the individual SG identified in the fluorescence micrograph shown in the right panel. Red, intensity before photobleach treatment; violet, 0.65 s after photobleach; blue, 22 s after photobleach. (right) Pseudocolor photomicrographs of COS transfectants before and 6 min after photobleaching. Arrows indicate the targeted SGs.