Table 1.
Altered hematopoiesis in Cdc42-deficient mice
| Parameters | MxCreTgcdc42+/+, n = 14 | MxCreTgcdc42f/f, n = 15 | CD45.1+ recipients reconstituted with MxCreTgcdc42+/+ bone marrow cells, n = 10 | CD45.1+ recipients reconstituted with MxCreTgcdc42f/f bone marrow cells, n = 10 |
|---|---|---|---|---|
| WBC, ×109/L | 4.3 ± 2.5 | 34.9 ± 10.0* ↑ | 12.0 ± 1.4 | 52.7 ± 11.6* ↑ |
| Neutrophils, ×109/L | 1.5 ± 0.8 | 29.3 ± 9.4* ↑ | 3.8 ± 0.7 | 41.1 ± 7.3* ↑ |
| Lymphocytes, ×109/L | 2.4 ± 1.9 | 3.5 ± 1.4 | 6.6 ± 1.9 | 7.8 ± 3.4 |
| Monocytes, ×109/L | 0.23 ± 0.16 | 0.67 ± 0.44† ↑ | 0.45 ± 0.19 | 1.49 ± 0.43† ↑ |
| Eosinophils, ×109/L | 0.09 ± 0.1 | 1.2 ± 0.7* ↑ | 0.14 ± 0.18 | 2.06 ± 0.33† ↑ |
| RBC, ×1012/L | 7.8 ± 0.9 | 7.2 ± 1.8 | 3.1 ± 4.4 | 4.3 ± 0.5 |
| Hb, g/L | 13.0 ± 2.0 | 10.9 ± 2.6‡ ↓ | 12.8 ± 1.5 | 8.2 ± 2.3‡ ↓ |
| HCT, % | 42.9 ± 4.8 | 38.5 ± 7.9‡ ↓ | 36.2 ± 3.4 | 24.6 ± 6.5‡ ↓ |
| RDW | 19.5 ± 1.0 | 26.2 ± 7.3† ↑ | 22.0 ± 0.1 | 29.0 ± 5.1‡ ↑ |
| Platelets, ×109/L | 903 ± 577 | 740 ± 202 | 455 ± 380 | 586 ± 442 |
Complete blood counts from primary Mx-CreTgcdc42f/f mice, WT mice engrafted with Mx-CreTgcdc42f/f bone marrow cells, and the corresponding control mice were performed at 14 to 19 DPI with polyI:C as described in Figure 1A,C. Hematologic measurements were performed on a Hemavet 850 Hematology Analyzer. The data are means plus or minus SD.
WBC indicates white blood cells; RBCs, red blood cells; Hb, hemoglobin; HCT, hematocrit; RDW, RBC distribution width; ↑, significant increase; and ↓, significant decrease.
*
P < .001.
†
P < .01.
‡
P < .05.