Table I.
Levels of metabolites in plant cytosol; PFP and H+-PPase activities from different plants and experimental conditions used in this study
Plants and Conditions
|
Metabolites in Plant Cytosola
|
Enzyme Activity Level
|
||||
---|---|---|---|---|---|---|
F1,6BP | Pi | Fru-6P | PPi | PFPb | H+-PPasec | |
mm | milliunits g fresh wt-1 | |||||
Wild-type plants | 0.6 | 5 | 1 | 0.33 | 293-1,813 | 15-25 |
Antisense transformant plants (lower levels) | - | - | - | - | 20-58 | - |
Experimental conditions (Figs. 1, 2, and 4) | - | - | - | - | 0.5-60d | 18-25d |
a The concentrations of F1,6BP, Pi, and F6P are those reported in the literature for plant cell cytosol (Rebeille et al., 1983; Ukaji and Ashihara, 1987; Weiner et al., 1987; Kubota and Ashihara, 1990; Davies et al., 1993; Stitt, 1998). b The PFP activities for wild-type and antisense transformant tobacco (Nicotiana tabacum) plants were taken from Nielsen and Stitt (2001) for antisense transformant potato (Solanum tuberosum) plants. Values are from Hajirezaei et al. (1994). c The H+-PPase activities for wild-type mung bean, tomato (Lycopersicon esculentum) fruit, and maize leaves were taken from Maeshima and Yoshida (1989), Milner et al. (1995), and Clayton et al. (1993), respectively. d The PFP and H+-PPase activities shown in this report were converted to milliunits per gram fresh wt-1 computing the enzyme activity level (milliunits) present in 1 mL of reaction medium. This volume was converted to grams to obtain the activities shown.