Histologic findings after in vivo transfer of LCL-13271 tumor cells in NOD/SCID mice. LCL-13271 injected intraperitoneally into NOD/SCID mice with tumor growth at 2 months in primary and secondary recipients. Abdominal tumor mass from NOD/SCID primary (A) and secondary (B) recipients of LCL-13271. Histologic findings were similar in morphology compared with the primary tumor (Huang et al35). Slides were viewed with an Olympus BX40 microscope (Olympus America) of sections stained with H&E medium (Hematoxylin Gill's Formulation no. 2, Fisher Diagnostics; Eosin-Y, Richard-Allan Scientific) using a lens at 400×. Images were acquired using a Hitachi charge-coupled device color camera (Hitachi Kokusai Electric America, Woodbury, NY) model HV-C20 3-CCD, and were processed with ACDSee version 4.0 software (ACD Systems International, Victoria, BC).