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. 2007 Aug 9;110(12):3996–4004. doi: 10.1182/blood-2007-02-074450

Figure 5.

Figure 5

Histologic findings after in vivo transfer of CML-14736 tumor cells into miniature swine. CML-14736 grew after in vivo transfer to histocompatible miniature swine pretreated with TBI. Tumor growth was found at the subcutaneous injection sites (A) and in the lungs after intravenous administration (B). Immunohistochemistry of the subcutaneous injection site tissue negative staining for CD3, but positive staining for CD16 and CD172a, which was consistent with the surface phenotype of the primary tumor and cultured cells (C). Slides were veiwed with a Nikon Eclipse E800 microscope (Nikon Instruments) of sections stained with H&E (Hematoxylin Gill's Formulation no. 2, Fisher Diagnostics; Eosin-Y, Richard-Allan Scientific) using Nikon Plan Fluor lenses at 400× (A right; C left, middle, right) and 200× (B). Images were acquired using Nikon HRD060-NIK 0.6X optical coupler diagnostic instruments (Nikon Instruments) connected to a computer with SPOT-diagnostic instruments and was processed with SPOT Advanced or Basic version 3.5.6 software for Windows (Diagnostic Instruments, Sterling Heights, MI).