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Determination of the molecular masses of the subunit (A) and native form (B), and of the pI (C) of M1PDH purified from the red alga C. continua. A, SDS-PAGE pattern. B, Molecular mass versus ratio of elution volume for the enzyme to the void volume (Ve/Vo) determined by gel filtration through a Sephacryl S-100 column. BSA, Bovine serum albumin; ova, ovalbumin; CA, carbonic anhydrase; myo, myoglobin. C, IEF pattern for the determination of pI.
