Figure 4.

pH profiles of M1PDH purified from the red alga C. continua. The activities were determined without NaCl (▪, F6P reduction; □, M1P oxidation), with 100 mm NaCl (•, F6P reduction; ○, M1P oxidation), or with 200 mm NaCl (▴, F6P reduction). A, 5 mm F6P and 0.5 mm M1P were respectively added as the substrate for the F6P-reducing and M1P-oxidizing reactions. B, F6P (0.5 mm) was added as the substrate for the F6P-reducing reaction. Final pH values are presented on the abscissa. The pH values did not change during the enzyme reaction.