Table I.
Purification of M1PDH from the red alga C. continua
The activity was determined by F6P reduction.
| Purification Step | Total Proteins | Total Activity | Specific Activity | |||
|---|---|---|---|---|---|---|
| mg | (%) | μmol min-1 | (—fold) | μmol min-1 mg-1 protein | (—fold) | |
| Crude extracta | 662 | (100) | 15 | (1) | 0.023 | (1) |
| PEG/AS treatmentb | 249 | (37.6) | 278 | (19) | 1.116 | (49) |
| Reactive Red 120 | 6.00 | (0.91) | 751 | (50) | 125.2 | (5,443) |
| BioAssist Q | 0.83 | (0.13) | 219 | (15) | 263.9 | (11,474) |
| Sephacryl S-100 | 0.70 | (0.11) | 160 | (11) | 228.6 | (9,939) |
a Activity was assayed after gel filtration through a Sephadex G-25 column (PD-10 column) equilibrated with buffer A as described in “Materials and Methods.” b Activity was assayed after gel filtration through a Sephadex G-25 column (2.6 × 90 cm) equilibrated with buffer A as described in “Materials and Methods.”