Table II.
Km and Vmax values for F6P reduction and M1P oxidation of purified M1PDH from the red alga, C. continua
| Substrate 1 | Substrate 2 | NaCl | Km for Substrate 1 | Vmax | |
|---|---|---|---|---|---|
| (mM) | mm | μm | μmol min-1 mg-1 protein | ||
| F6P reduction | |||||
| F6P | NADH | (0.2) | 0 | 188.8 | 128.8 |
| F6P | NADH | (0.2) | 200 | 1458 | 164.4 |
| NADH | F6P | (5.0) | 0 | 3.1 | 22.5 |
| NADH | F6P | (5.0) | 200 | 17.8 | 96.0 |
| NADH | F6P | (0.5) | 0 | 3.5 | 47.5 |
| NADH | F6P | (0.5) | 200 | 5.4 | 15.2 |
| M1P oxidation | |||||
| M1P | NAD | (0.2) | 0 | 48.9 | 33.2 |
| M1P | NAD | (0.2) | 100 | 138.0 | 40.2 |
| NAD | M1P | (0.5) | 0 | 32.0 | 34.0 |
| NAD | M1P | (0.5) | 100 | 56.9 | 38.3 |