Table III.
Comparison of M1PDH properties in various organisms
–, No data. NA, No activity. Red, Reductive activity. Ox, Oxidative activity. G6P, Glc-6-phosphate. Gluci6P, glucitol-6-phosphate. S6P, sorbitol-6-phosphate; underlined values indicate testing with a crude or partially purified preparation.
| Molecular
|
Km
|
Optimum pH
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Source | Native
|
Subunit
|
Structure | Red
|
Ox
|
Red
|
Ox
|
Substrate Specificity (Reacted with) | Inhibitor | ||
| F6P | NADH | M1P | NAD | ||||||||
| kD | μm | ||||||||||
| Red alga | |||||||||||
| C. continuaa | 53 | 53 | 1-mer | 147 | 3 | 51 | 26 | 7.2 | 9.0 | Narrow | NEM, pHMB |
| 1,326p | 23p | 133q | 45q | Zn2+ | |||||||
| C. leprieuriib | - | - | - | 1,400 | 20 | 90 | 23 | 7.0 | 8.5 | Wide (G6P) | - |
| Brown alga | |||||||||||
| S. pacificumc | - | - | - | 280 | 13 | 250 | 71 | 6.5 | 10.2 | Wide (G6P, Man6P) | pHMB, Hg2+ ATP, ADP |
| Green alga | |||||||||||
| P. subcordiformisd | - | - | - | 5,500 | - | NA | NA | 7.5 | NA | Narrow | - |
| Fungus | |||||||||||
| A. nigere | 40 | 22 | 2-mer | 540 | 5 | 38 | 83 | - | - | - | - |
| A. parasiticusf | - | - | - | - | - | - | - | - | - | - | Zn2+, Cd2+, Co2+, Ni2+ |
| C. neoformansg | 148 | 36 | 4-mer | 300 | 99 | 55 | 110 | 7.0-7.5 | 9.0-9.5 | Wide (S6P, G6P, EtOH, acetaldehyde) | - |
| S. sclerotiorumh | - | - | - | - | - | - | - | 7.5 | 10.5 | Wide (G6P, Gluci6P) | pHMB, Hg2+, NEM (Ox) |
| Apiconplexa | |||||||||||
| E. tenellai | - | 67 | Hetero-trimero | - | - | - | - | - | - | Wide (NADP, NADPH) | - |
| Bacterium | |||||||||||
| A. aerogenesj | 40 | - | - | 2,100 | 140 | 2,000 | 380 | - | 9.5 | Narrow | - |
| B. thermosphactak | 50 | 50 | 1-mer | 250 | - | 50 | - | 6.5 | 9.5 | - | ATP (Ox), ADP (Ox) |
| E. colil | 47 | 45n | 1-mer | 200 | 10 | 200 | 200 | 7.0 | 9.5 | Narrow | pHMBr |
| S. mutansm | 45 | - | - | 1,660 | 16 | 150 | 66 | 6.5 | 8.5-9.0 | Narrow | ADP |
a Present work. b Karsten et al. (1997a). c Ikawa et al. (1972). d Richter and Kirst (1987). e Kiser and Niehaus (1981). f Forema n and Niehaus (1985). g Suvarna et al. (2000). h Wang and Le Tourneau (1972). iSchmatz (1997). jLiss et al. (1962). k Singh and Rogers (1993). l Novontny et al. (1984). mBrown and Bowles (1977). nTeschner et al. (1990). oOne catalytic protein and two 35-kD 14-3-3 proteins. p Assayed in the presence of 200 mm NaCl. q Assayed in the presence of 100 mm NaCl. r Wolff and Kaplan (1955).