Table 1.

Mean dimensions and number of vesicles and grains of Bassoon-or Piccolo-labeled transport aggregates in young axons 3-6 day old^* measured from single sections.

proteins	Mean# dimensions of aggregates (μm)	Number of DCV	Number of clear vesicles	Number of grains
Bassoon (47 aggregates from 2 experiments**)	0.217 ± 0.007 by 0.128 ± 0.003	1.4 ± 0.2	4.9 ± 0.3	7.0 ± 0.4
Piccolo (rabbit antibody, 21 aggregates from 3 experiments)	0.216± 0.010 by 0.129 ± 0.005	1.2 ± 0.2	5.6 ± 0.5	8.2 ± 0.9
P value	0.97, 0.89	0.48	0.23	N. A.ˆ

^*Quantitative measurements were taken from 3-6 day old cultures because aggregates were relatively scarce in older cultures and tend to have more SV-like vesicles (cf. Supplemental Fig. 1A1-A4) than those in younger cultures.

^#Mean ± SEM

^**Measurements from different experiments were only pooled when the labeling intensities were similar.

^ˆLabeling intensities were not compared among different antibodies.