Figure 3.

Carbohydrate content in wild-type, sense (S3 and S7), and antisense (150–91) lines 2 h into the photoperiod. Sugar content was determined by HPLC-pulsed amperometric detection. Data are from two replicated experiments with three replicates for each genotype. Bars represent the mean ± sem; n = 6; nonacclimated (white bars); cold-acclimated (black bars): 15°Cfor7d, 10°Cfor7d, 5°Cfor7d, and 3°C for 3 d. Starch was determined enzymatically and is estimated as micromoles of Glu per gram of dry weight.