Table I.

Raffinose content in nonacclimated and cold-acclimated sink and source leaves of wild-type and transgenic petunia lines

Treatment	Genotype	Raffinose Content
		Sink leaves	Source leaves
		μmol g-1 dry weight
Nonacclimated	WT	0.23 ± 0.18	0.53 ± 0.11
	S3	0.22 ± 0.17	0.46 ± 0.13
	S7	0.23 ± 0.18	0.50 ± 0.11
	150	16.69 ± 0.29	19.87 ± 0.36
	149	18.47 ± 0.62	24.98 ± 0.42
	147	16.39 ± 0.64	22.54 ± 0.34
	146	15.22 ± 0.68	18.98 ± 0.22
	105	21.78 ± 0.43	26.67 ± 0.32
	91	12.23 ± 0.46	15.46 ± 0.23
Cold acclimated	WT	5.91 ± 0.82	5.00 ± 0.88
	S3	0.71 ± 0.10	0.66 ± 0.21
	S7	0.70 ± 0.12	0.77 ± 0.67
	150	24.30 ± 0.26	22.97 ± 0.55
	149	52.34 ± 0.23	50.46 ± 0.13
	147	35.60 ± 1.15	33.77 ± 0.82
	146	26.84 ± 1.02	23.41 ± 0.57
	105	52.25 ± 0.62	49.89 ± 0.66
	91	21.28 ± 0.52	20.54 ± 0.35

Sense lines (S3 and S7); antisense lines (150, 149, 147, 146, 105, and 91).

Petunia plants were nonacclimated (22°C, 16/8-h photoperiod) or gradually cold acclimated from 15°C to 3°C, 12/12-h photoperiod for 24 d. Sink leaves (-2 cm²) from the top one-half of the plants and source leaves (-4.5 cm²) from the bottom one-half of the plants were sampled 2 h into the photoperiod. Raffinose content was determined by HPLC-PAD. Data are from two replicated experiments with three replicates for each genotype.

Values are mean ± sem; n = 6.