Figure 3.

Estimation of CBF transcript half-life at warm temperatures. Plants that had been exposed to a low temperature (4°C) for 2 h were returned to a warm temperature (24°C) and CBF transcript levels were determined at 10-min intervals by RNA-blot hybridization. Air and agar temperatures in a plate were recorded during the experiment. Transcript levels for eif-4A did not change significantly during the time course and were used as a reference for loading. The probe for CBF hybridized with CBF1, 2, and 3 transcripts.