Figure 8.

Sequences of the CBF2 promoter involved in cold, cycloheximide, and mechanical responsiveness. Plants containing CBF2 promoter constructs were grown at 22°C (W) and then exposed to 4°C (C) for 2 h or treated with cycloheximide (X) or mechanical agitation (M) as described in “Materials and Methods.” RNA was isolated and GUS transcript levels were determined by RNA-blot hybridization. A, Plants containing the 5′ deletion to –189 (5′Del) or the –189/–65 dimer (Dimer) construct. B, Plants containing the m2, m3, m4, and mboxIV mutations. rRNA stained with ethidium bromide was used to compare loading in each experiment.