Table I.

Probes, primers, and their final optimal concentrations used in real-time PCR

Each Taqman probe was labeled with 6-fluorescein at the 5′ end and the quencher TAMRA at the 3′ end.

Enzyme	Primers/ Probes	Sequence (5′ to 3′)	Optimal Concentration
			nm
HPT	Forward	TCTCTAAAAGACTTCTGTTTGCTATTCG	900
	Reverse	AGTCGAGATTTCGGGTTAATGC	900
	Probe	AAAGCCTCAGGCTGACCCGCAGT	175
HPPD	Forward	CAAGGAGTGTGAGGAATTAGGGATT	300
	Reverse	TCGTCGGCCTGTCACCTAGT	300
	Probe	ATGATCAAGGGACGTTGCTTCAAATCTTCA	150
HGAD	Forward	ACCAACCATCGAGGAGGAAA	900
	Reverse	TAAATACAGTCCTTACAGCACCAACTG	900
	Probe	ATGACAAAATCAAGCAAGGCCACACCA	175
TC	Forward	GTATTTGAGCCTCATTGGCAGAT	900
	Reverse	TCACCGCCCCATTCTATCC	900
	Probe	ATGGCAGGAGGCCTTTCCACAGG	200
GGPS1	Forward	TCCGGTGAGAGTGGTTCGAG	900
	Reverse	CTTGACCCGCCACTAACCC	900
	Probe	TTGGAGAATTGGCTAAAGCGATAGGAACAGA	200
GGDR	Forward	CACTTAGGGAACACCCAACCA	900
	Reverse	ACTTACTATGAGGATTTAGCTGAGATGTATG	900
	Probe	AGAAATCCGGCGACACATCATCTCCA	150
TAT	Forward	AACCGAAAGCCAACGTTTTG	900
	Reverse	TCTTGTAGATGGAGCGGACTAGGT	600
	Probe	TTCCGAGTCCCGGCTTCCCATG	200
g-TMT	Forward	GGCCAAGAGAGCCAATGATC	900
	Reverse	CGCATCCGCAACTTGGA	900
	Probe	CGGCTGCTCAATCACTCTCTCATAAGGCT	200
EF-1α	Forward	CGAACTTCCATAGAGCAATATCGA	300
	Reverse	GCATGGGTGTTGGACAAACTT	300
	Probe	ACCACGGTCACGCTCGGCCT	175