Figure 4.

The EMSA (A) and DMS footprinting (B) of intramolecular G-quadruplex structures of PDGF-A Pu48-mer. In (A), high-mobility bands 1 and 2 represent compact intramolecular G-quadruplex structures, and band 3 represents linear DNA, which was fully denatured by heating. In (B), DMS footprinting of band 1 (lanes 1 and 2), band 2 (lanes 4 and 5) and band 3 (lane 3) from (A) is shown. Open circles indicate the guanines that are fully protected, partially open circles indicate the guanines that are partially protected and arrowheads indicate the guanines that are cleaved.