Figure 3.

Ebs1p and Upf1p co-localize in P-bodies in glucose-starved cells. Yeast cells expressing Ebs1p-dsRED and Dcp2p-GFP fusions as well as cells expressing Ebs1p-dsRED and Upf1p-GFP were grown in rich synthetic media and successively resuspended in media lacking glucose while shaking at 30°C. Four hours after, confocal images of live cells were taken. Arrows point to cytoplasmic foci where a co-localization between Ebs1p-dsRED with Dcp2p-GFP and Ebs1p-dsRED with Upf1p-GFP occurred. Scale bar represents 2.5 μm.