Figure 4.

Overexpression of Ebs1 recruits Upf1p into P-bodies. Upf1p-GFP and Dcp2p-RFP cells were transformed with either empty vector (EV) or with a plasmid, which overexpressed Ebs1p under the ADH promoter (ADH-Ebs1) and subjected to confocal microscopy. Arrowheads indicate examples of cytoplasmic foci where a co-localization between Upf1p-GFP and Dcp2p-RFP occurred. At the time of imaging, 74% of the cells transformed with ADH-Ebs1 displayed 2–6 Upf1p-GFP foci and 6% of the cells transformed with empty vectors displayed 1 to 2 Upf1p-GFP foci. Scale bar represents 2.5 μm.