Table 3.
Nucleotide | Nucleotide | |||
---|---|---|---|---|
Gene/Region | |
AA Change | ||
Position | WTa | SPb | ||
625 | Pr-54 | C | U | Proline→Serine |
1599 | E211 | C | U | |
3707 | NS2A-61 | U | C | Valine→Alanine |
9660 | NS5 | C | U |
WT: WNV 3356K isolated from the kidney of an American crow collected in New York in 2000, and amplified twice in Vero cells
SP: A small plaque variant (SP), isolated from the first Vero amplified WT stock, followed by 2 sequential rounds of plaque purification and two rounds of amplification in Vero cells.