. Author manuscript; available in PMC: 2008 Oct 25.

Published in final edited form as: Virology. 2007 Jul 6;367(2):339–347. doi: 10.1016/j.virol.2007.06.008

Table 4.

Sequence analysis of revertant viruses derived from SP-infected mosquitoes or cells

Source of virus	Incubation temperature	Plaques picked	prM 625 (U to C) reversion	NS2A 3707 (C to U) reversion
2 Mosquitoes	15°C	10 lp^a	6^c	4^c
		10 sp^b	5	2
2 Mosquitoes	27°C	10 lp	10	0
		10 sp	4	0
2 Mosquitoes	32°C	10 lp	10	0
		10 sp	5	0
Vero	42.5°C	5 lp	5	0
DF-1	42.5°C	5 lp	5	0

Ten-fold serial dilutions of virus recovered from SP infected mosquitoes and Vero or DF-1 cells were plated on Vero cell monolayers in 6-well plates. Well-isolated plaques were picked from the wells containing only 1 or 2 plaques at 72 hpi.

lp: Plaque diameter equal to or larger than 1mm

sp: Plaque diameter smaller than 1mm

Includes one large plaque with double reversion