Table 1.
Primer pairs used for the amplification of genes involved in mammalian sex determination
| Sequence | |
|---|---|
| DMRT1 exon 1 | |
| Forward | GGCAGACCTCGCCACTCCAG |
| Reverse | AAGGCTGAACCCGGGCTCCC |
| DMRT1 exon 2 | |
| Forward | TCTGTGTTTTGGCAAAGCTG |
| Reverse | CTGCTTCTGTGGCTGCAA |
| DMRT1 exon 3 | |
| Forward | GCAGGTCTTGGGTAGGAAGG |
| Reverse | CATGTGGCTTTCACACAACC |
| DMRT1 exon 4 | |
| Forward | CAAGGTGTCGGGAACATAGG |
| Reverse | CTCTCTCAACCCCAAATCCA |
| DMRT1 exon 5 | |
| Forward | GGAGAGCGTCACTTTCTTTGTT |
| Reverse | CCATGCAGATGGTAGTCACG |
| DMRT3 exon 1 | |
| Forward | CGGAGCACACACGACCAC |
| Reverse | GTCCTCCCAAGTGGAGCTG |
| DMRT3 exon 2 | |
| Forward 1 | TGCATTTGCTCTTCCAAAA |
| Reverse 1 | AGAGTCGGCAGAAAACCTCA |
| Forward 2 | AACTTCCGCAGAACCTGAGA |
| Reverse 2 | AGATGTGGCCTCTCCTCAGA |
| BPESC1 | |
| Forward | AAGGTGACTTAAGGGCAGAGC |
| Reverse | GCCTGTCTCCAGACAAGAGTG |
| WNT4 exon 1 | |
| Forward | CCCAGGTAACCCCATCCT |
| Reverse | GGTGTGCAGAGGGACGTT |
| WNT4 exon 2 | |
| Forward | ACAGCATTTCCACTCCCTTG |
| Reverse | TCCTTTATGCCCTCACTTGG |
| WNT4 exons 3/4 | |
| Forward | GGGTGCCTAGCACATGATTT |
| Reverse | TGAGAGCCTGCACAAATGTT |
| WNT4 exon 5 | |
| Forward | CACAACGGCAAATCTGACTG |
| Reverse | TGAGGACCCAAAAACCAAAC |
| DAX1 exon 1 | |
| Forward 1 | ACAGCATCCAGGACATAGTGG |
| Reverse 1 | TGCCTCCTGGGACCTATTTAT |
| Forward 2 | CGTGCGCGCTAGGTATAAAT |
| Reverse 2 | AAGCAGCAGCGGTACAGAAG |
| Forward 3 | ACTAGCTCAAAGCAAACGCAC |
| Reverse 3 | TCCTCTTGGCTGAGTTTCTGA |
| DAX1 exon 2 | |
| Forward 1 | AGCAAAGGACTCTGTGGTGAG |
| Reverse 1 | GCAGGTTCCATGAAATTGCTA |
| TSPYL | |
| Forward | GCCGCTGAAATGTTAGTGAGA |
| Forward | GGAAACAGGGTFCAGAAAAG |
| SOX9 exon1 | |
| Forward | GCGCCTTCCTAAGTGCTC |
| Reverse | GCAAATCAGCCCTGACCA |
| SOX9 exon 2 | |
| Forward | TGACCCCTCTCCCTCTTTTT |
| Reverse | TGCCTCTTAGGCTCTGGGTA |
| SOX9exon3 | |
| Forward 1 | GCACAGCCCTTGTTGATTTT |
| Reverse 1 | CTCAGCTGCTCCGTCTTGAT |
| Forward 2 | ATCAAGACGGAGCAGCTGAG |
| Reverse 2 | AGCGAACGCACATCAAGAC |