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The effect of different amino acid substitutions at the upper hinge residues (T154 in Kir4.1 subunit and S157 in Kir5.1 subunit) on single-channel open-probability (Po) of heteromeric Kir4.1/Kir5.1 channels. WT wild-type Kir4.1/Kir5.1. Currents were measured in the cell-attached mode at −120 mV, n = 6. *P < 0.005
