Figure 3.

Blocking the lysis of 1106 mel target cells is proportional to the amount of mAb added. NK clone 10 (CD56⁺, CD16⁺, NKIR2⁺, NKIR1⁻, NKB1⁻, CD94⁺) was incubated with various concentrations of F(ab′)₂ fragment of either the anti-CD16 mAb 3G8 or the anti-CD99 mAb 12E7 for 1 hr on ice. The NK clone 10 incubated with no mAb was used as a control. Cells were washed and incubated with the ³⁵S-labeled target cells at an E:T ratio of 5:1 in a 5-hr killing assay. The figure shows one representative experiment of three performed.