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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Aug;163(2):756–762. doi: 10.1128/jb.163.2.756-762.1985

Genetic location of genes encoding enterobacterial common antigen.

U Meier, H Mayer
PMCID: PMC219186  PMID: 3894334

Abstract

A new rff mutation (rff-726) of Escherichia coli is described which affects the biosynthesis of the enterobacterial common antigen. This mutation was detected in an rfe-defective strain. A Tn10 insertion near the rfe locus was isolated to facilitate further mapping. Both mutations rfe and rff were mapped by transduction with bacteriophage P1, giving the gene order ilv rfe rff uvrD metE. The F' factor F14 was able to complement both mutations rfe and rff, whereas the F' factor F16 could complement the rfe but not the rff mutation. The rff mutation did not affect the biosynthesis of N-acetyl-D-mannosaminuronic acid, as the previously described rff mutations in Salmonella typhimurium do (H. C. Lew, H. Nikaido, and P. H. Mäkelä, J. Bacteriol. 136:227-233, 1978), and also did not affect the biosynthesis of other enterobacterial common antigen components; however, the biosynthesis of the complete enterobacterial common antigen molecule was blocked.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun V., Gnirke H., Henning U., Rehn K. Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope. J Bacteriol. 1973 Jun;114(3):1264–1270. doi: 10.1128/jb.114.3.1264-1270.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooper P. H., Hirshfield I. N., Maas W. K. Map location of arginyl-tRNA synthetase mutations in Escherichia coli K-12. Mol Gen Genet. 1969 Aug 15;104(4):383–390. doi: 10.1007/BF00334238. [DOI] [PubMed] [Google Scholar]
  4. Jann B., Jann K. 4-amino-4,6-dideoxyhexoses isolated from lipopolysaccharides of Escherichia coli. Eur J Biochem. 1967 Jul;2(1):26–31. doi: 10.1111/j.1432-1033.1967.tb00100.x. [DOI] [PubMed] [Google Scholar]
  5. Jann K., Schmidt G., Wallenfels B., Freund-Molbert E. Isolation and characterization of Escherichia coli bacteriophage omega-8 specific for E. coli strains belonging to sero-group O 8. J Gen Microbiol. 1971 Aug;67(3):289–297. doi: 10.1099/00221287-67-3-289. [DOI] [PubMed] [Google Scholar]
  6. Kleckner N., Barker D. F., Ross D. G., Botstein D. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics. 1978 Nov;90(3):427–461. doi: 10.1093/genetics/90.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuhn H. M., Neter E., Mayer H. Modification of the lipid moiety of the enterobacterial common antigen by the "Pseudomonas factor". Infect Immun. 1983 May;40(2):696–700. doi: 10.1128/iai.40.2.696-700.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee H. J., Otsubo E., Deonier R. C., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv+ Deletion mutants of F14. J Mol Biol. 1974 Nov 15;89(4):585–597. doi: 10.1016/0022-2836(74)90037-0. [DOI] [PubMed] [Google Scholar]
  9. Lew H. C., Nikaido H., Mäkelä P. H. Biosynthesis of uridine diphosphate N-acetylmannosaminuronic acid in rff mutants of Salmonella tryphimurium. J Bacteriol. 1978 Oct;136(1):227–233. doi: 10.1128/jb.136.1.227-233.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lüderitz O., Galanos C., Lehmann V., Mayer H., Rietschel E. T., Weckesser J. Chemical structure and biological activities of lipid A's from various bacterial families. Naturwissenschaften. 1978 Nov;65(11):578–585. doi: 10.1007/BF00364907. [DOI] [PubMed] [Google Scholar]
  11. MATSUHASHI M., STROMINGER J. L. THYMIDINE DIPHOSPHATE 4-ACETAMIDO-4, 6-DIDEOXYHEXOSES. I. ENZYMATIC SYNTHESIS BY STRAINS OF ESCHERICHIA COLI. J Biol Chem. 1964 Aug;239:2454–2463. [PubMed] [Google Scholar]
  12. Mayer H. D-Mannosaminuronsäure-Baustein des K7-antigens von Escherichia coli. Eur J Biochem. 1969 Mar;8(1):139–145. doi: 10.1111/j.1432-1033.1969.tb00506.x. [DOI] [PubMed] [Google Scholar]
  13. Mayer H., Schmidt G. Chemistry and biology of the enterobacterial common antigen (ECA). Curr Top Microbiol Immunol. 1979;85:99–153. doi: 10.1007/978-3-642-67322-1_3. [DOI] [PubMed] [Google Scholar]
  14. Mäkelä P. H., Jahkola M., Lüderitz O. A new gene cluster rfe concerned with the biosynthesis of Salmonella lipopolysaccharide. J Gen Microbiol. 1970 Jan;60(1):91–106. doi: 10.1099/00221287-60-1-91. [DOI] [PubMed] [Google Scholar]
  15. Mäkelä P. H., Mayer H. Enterobacterial common antigen. Bacteriol Rev. 1976 Sep;40(3):591–632. doi: 10.1128/br.40.3.591-632.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mäkelä P. H., Mayer H., Whang H. Y., Neter E. Participation of lipopolysaccharide genes in the determination of the enterobacterial common antigen: analysis of R mutants of Salmonella minnesota. J Bacteriol. 1974 Sep;119(3):760–764. doi: 10.1128/jb.119.3.760-764.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mäkelä P. H., Schmidt G., Mayer H., Nikaido H., Whang H. Y., Neter E. Enterobacterial common antigen in rfb deletion mutants of Salmonella typhimurium. J Bacteriol. 1976 Sep;127(3):1141–1149. doi: 10.1128/jb.127.3.1141-1149.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Männel D., Mayer H. Isolation and chemical characterization of the enterobacterial common antigen. Eur J Biochem. 1978 May 16;86(2):361–370. doi: 10.1111/j.1432-1033.1978.tb12318.x. [DOI] [PubMed] [Google Scholar]
  19. Otsubo E., Deonier R. C., Lee H. J., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IV. The F sequences in F14. J Mol Biol. 1974 Nov 15;89(4):565–584. doi: 10.1016/0022-2836(74)90036-9. [DOI] [PubMed] [Google Scholar]
  20. PITTARD J., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. II. INTERACTION BETWEEN F-MEROGENOTE AND CHROMOSOME DURING TRANSFER. J Bacteriol. 1963 Jun;85:1402–1408. doi: 10.1128/jb.85.6.1402-1408.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pang P. P., Walker G. C. Identification of the uvrD gene product of Salmonella typhimurium LT2. J Bacteriol. 1983 Mar;153(3):1172–1179. doi: 10.1128/jb.153.3.1172-1179.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sarvas M. Mutant of Escherichia coli K-12 defective in D-glucosamine biosynthesis. J Bacteriol. 1971 Feb;105(2):467–471. doi: 10.1128/jb.105.2.467-471.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schmidt G. Konjugation bei E. coli O8:K27(A): H minus. Zentralbl Bakteriol Orig. 1969;211(3):335–344. [PubMed] [Google Scholar]
  24. Schmidt G., Mayer H., Mäkelä P. H. Presence of rfe genes in Escherichia coli: their participation in biosynthesis of O antigen and enterobacterial common antigen. J Bacteriol. 1976 Aug;127(2):755–762. doi: 10.1128/jb.127.2.755-762.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wechsler J. A., Adelberg E. A. Antipolarity in the ilv operon of Escherichia coli K-12. J Bacteriol. 1969 Jun;98(3):1179–1194. doi: 10.1128/jb.98.3.1179-1194.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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