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. 1999 May 11;96(10):5674–5679. doi: 10.1073/pnas.96.10.5674

Figure 1.

Figure 1

Expression of CD94/NKG2A and LIR-1 in a NK-enriched cell line derived from a pregnant woman and partial protection from lysis mediated by HLA-G1 expression on target cells. (A) Double-fluorescence analysis of a CD3-depleted (NK-cell enriched) lymphocyte population cultured for 8 days in the presence of rIL-2. Cells were stained with different combinations of the following mAbs: anti-CD56, anti-CD16, anti-CD94, anti-NKG2A, and anti-LIR-1 (x axis, green fluorescence; y axis, red fluorescence). (B) The same NK-enriched cultured lymphocyte population was analyzed for the ability to lyse 221 cells or 221-G1 cell transfectants. In addition, the cytolytic activity against 221-G1 was assessed also in the presence of Y9 (anti-CD94) mAb, M401 (anti-LIR-1), or a combination of the two mAbs. Each histogram represents the mean of triplicate experiments.