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. 1999 May 11;96(10):5674–5679. doi: 10.1073/pnas.96.10.5674

Figure 2.

Figure 2

Specific binding of the anti-p49 TOP-2 antiserum to p49 cell transfectants. (A) Binding of p49-Fc soluble molecule to HLA-G1 cell transfectants. p49-Fc has been incubated with HLA-G1 221-transfected cells (Left) or with 221 untransfected cells (Right) followed by anti-human IgG PE-conjugated mAb. White profiles represent controls stained with the second reagent only. (B) Staining of cl.15.212-transfected COS-7 cells by the anti-p49 TOP-2 antiserum. COS-7 cells transfected with cl.15.212-VR1012 construct (Left) or with an irrelevant cDNA (Right) were stained with TOP-2 antiserum followed by a PE-conjugated anti-mouse IgG1. White profiles represent controls stained with the second reagent only.