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. 1999 May 11;96(10):5680–5685. doi: 10.1073/pnas.96.10.5680

Figure 1.

Figure 1

Time- and replication-dependent induction of RANTES expression after RSV infection in airway epithelial cells. Epithelial (hTBE) cell monolayers were uninfected (time 0) or infected with RSV or RSV rendered nonreplicative by UV irradiation (RSV-UV) at an MOI of 1.0 (or equivalent for RSV-UV) for 4–48 h, and total cellular RNA (20 μg/lane) was subjected to Northern blot analysis with RANTES, IRF-1, ICAM-1, IL-8, and β-actin cDNAs, and corresponding mRNAs were detected by autoradiography. Comparative responses to IFN-γ (100 units/ml for 24 h, lane 9) are shown as a positive control for IRF-1 expression. No signal was detected after hybridization with cDNAs for other β-chemokines: MCP-1, MCP-3, MIP-1α, or MIP-1β, or eotaxin (data not shown). Comparative responses for IL-8 mRNA served as a positive control for RSV-UV effect. Results are representative of five experiments.