Figure 3.

Analysis of RSV-dependent increases in RANTES expression by mRNA half-life assay. Uninfected and RSV-infected (MOI 1.0 for 24 h) hTBE cell monolayers were concomitantly pulsed with ³H-uridine to label cellular RNA and then chased in medium containing unlabeled uridine and cytidine for the indicated times before isolating total cellular RNA. Radiolabeled RANTES was captured by blot hybridization with RANTES antisense cRNA. (A) The level of RANTES transcript at each time point was calculated as dpm bound to antisense cRNA minus dpm bound to sense cRNA corrected for total dpm in each sample. (B) These values were expressed as a percentage of the initial level at the start of the chase period (time 0) to calculate mRNA half-life.