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. 2001 Jun 11;153(6):1199–1208. doi: 10.1083/jcb.153.6.1199

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The centromere is assembled during prophase. Nuclei from wild-type (A–C), hcp-3(RNAi) (D–F), and hcp-4 (RNAi) (G–I) embryos were fixed and stained with DAPI (blue), α–HCP-3 pAb (red), and α–HCP-1 (green). A P0 hcp-3(RNAi) embryo, postpronuclei fusion, stained with DAPI (J), α–HCP-3 pAb (K), and α–HCP-4 pAb (L). (M) Diagram summarizing the order of assembly for the centromere–kinetochore complex. Bar, 5 μm.