Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 Jun 11;153(6):1199–1208. doi: 10.1083/jcb.153.6.1199

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Sister centromere resolution. Individual chromosomes from prophase nuclei were stained with DAPI (blue), α–HCP-1 antibody (not shown), and α–HCP-3 antibody (red). Nuclei containing little or no HCP-1 staining were optically sectioned and a three-dimensional projection generated using the volume viewer function. The resulting images were rotated around the Y-axis. (A) Chromosomes showing different degrees of sister centromere separation. Cartoon of the Z-axis of each chromosome depicting the sister centromeres migration from a juxtaposed position to a maximally resolved orientation is shown adjacent to each image. (B) A single chromosome showing an intermediate “splitting” of sister centromeres. (C and D) Sister centromere resolution is a microtubule-independent process. (C) P1 blastomere from a wild-type embryo stained with DAPI (blue), antitubulin antibody (green), and α–HCP-3 antibody (red). (D) P1 blastomere, from an embryo incubated in the presence of 30 μg/ml nocodazole at room temperature for 30 min, stained as in C. The division time for a two-cell embryo at room temperature is ∼20–30 min, so this embryo had probably just finished cell division when treated with nocodazole. Furthermore, the centrosomes are duplicated (not shown) but have not migrated, indicating that loss of spindle microtubules occurred before centrosome migration, an event that occurs concurrent or before sister centromere resolution (C). Bar, 5 μM.

Sister centromere resolution. Individual chromosomes from prophase nuclei were stained with DAPI (blue), α–HCP-1 antibody (not shown), and α–HCP-3 antibody (red). Nuclei containing little or no HCP-1 staining were optically sectioned and a three-dimensional projection generated using the volume viewer function. The resulting images were rotated around the Y-axis. (A) Chromosomes showing different degrees of sister centromere separation. Cartoon of the Z-axis of each chromosome depicting the sister centromeres migration from a juxtaposed position to a maximally resolved orientation is shown adjacent to each image. (B) A single chromosome showing an intermediate “splitting” of sister centromeres. (C and D) Sister centromere resolution is a microtubule-independent process. (C) P1 blastomere from a wild-type embryo stained with DAPI (blue), antitubulin antibody (green), and α–HCP-3 antibody (red). (D) P1 blastomere, from an embryo incubated in the presence of 30 μg/ml nocodazole at room temperature for 30 min, stained as in C. The division time for a two-cell embryo at room temperature is ∼20–30 min, so this embryo had probably just finished cell division when treated with nocodazole. Furthermore, the centrosomes are duplicated (not shown) but have not migrated, indicating that loss of spindle microtubules occurred before centrosome migration, an event that occurs concurrent or before sister centromere resolution (C). Bar, 5 μM.