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. 2001 Jun 11;153(6):1187–1198. doi: 10.1083/jcb.153.6.1187

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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(A) Structure and expression of the ICFP. (B) Yeast expressing ICFP either in single copy (YIp), low copy (CEN plasmid), or high copy (2 μ plasmid) were grown as patches and replica plated onto nitrocellulose filters atop galactose-containing plates. After 40 h, the filters were washed to remove cells and immunoblotted with antiinsulin antibody and a peroxidase-conjugated secondary antibody.

(A) Structure and expression of the ICFP. (B) Yeast expressing ICFP either in single copy (YIp), low copy (CEN plasmid), or high copy (2 μ plasmid) were grown as patches and replica plated onto nitrocellulose filters atop galactose-containing plates. After 40 h, the filters were washed to remove cells and immunoblotted with antiinsulin antibody and a peroxidase-conjugated secondary antibody.