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. 2001 Jun 11;153(6):1187–1198. doi: 10.1083/jcb.153.6.1187

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Structural maturation of insulin upon Kex2p-mediated endoproteolysis. (A) Sucrose velocity gradient centrifugation. (B) Disulfide accessibility of ICFP and mature insulin under denaturing and nondenaturing conditions. Note that in these experiments all samples were exposed to PNGase F (A) or endoglycosidase H (B) at the end of the analysis. In the experiment shown in B, the enzyme digestion was performed in the complete absence of a reducing agent. The positions of reduced ICFP and insulin standards (ICFP-R and INS-R) and oxidized standards (ICFP-O and INS-O) are shown to the left of the panel.