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Indirect immunofluorescence localization of insulin in a pep4 strain and a vps4 pep4 double mutant. Note that insulin accumulates in the vacuole in a pep4Δ mutant and in the prevacuolar compartment in the class E vps mutant. Cells were fixed, permeabilized, and insulin labeled with antiinsulin and a Cy3-conjugated secondary antibody.
