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. 2001 Jun 11;153(6):1151–1160. doi: 10.1083/jcb.153.6.1151

Figure 3.

Figure 3

Far ultraviolet CD spectra of Tom40, TOM core complex, and mitochondrial porin isolated from N. crassa mitochondria. (A) Tom40; (B) TOM core complex; and (C) porin. Protein was solubilized in 0.1% DDM, 50 mM potassium-acetate/MOPS, pH 7.0, 10% glycerol. 10 scans were accumulated at 4°C. The protein concentrations of Tom40 (2.5 μM) and mitochondrial porin (6.9 μM) relevant for computing the molar ellipiticities ΘR were determined by UV absorbance spectroscopy. The protein concentration of the TOM core complex was determined using a colorimetric assay.