Table 1.
Comparison of the CD Spectrum of Tom40 with that of TOM Core Complex and Mitochondrial Porin
Protein | λ crossover | λ min | ΘR min | α-Helix | β-Sheet + turn | Other |
---|---|---|---|---|---|---|
nm | 10−3 deg cm2 dmol−1 | % | % | % | ||
Tom40 | 202 | 216.5 | −11.7 | 32 | 22 | 46 |
TOM core complex | 202 | 210 and 226 | −5.6 | ND | ND | ND |
VDAC (mitochondrial porin) | 206 | 216.0 | −3.4 | 15 | 46 | 34 |
The secondary structure estimates were computed from CD spectra of Tom40 and mitochondrial porin of N. crassa in 0.1% DDM as described in Materials and Methods. The content of α-helix and β-sheet was estimated for Tom40 and mitochondrial porin only, since the protein concentration of the TOM core complex could not be determined with the required accuracy.