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. 2001 Jun 11;153(6):1287–1300. doi: 10.1083/jcb.153.6.1287

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Overexpression of CLIMP-63 results in formation of ER clusters in restricted areas of the cytoplasm. COS cells were transiently transfected with wt-GFP and subjected to ultrathin cryosectioning followed by immunolabeling with anti-GFP and protein A–gold. (A) The cell at the left expresses high levels of wt-GFP as judged by the high density of gold particles and displays clustered ER (arrows). The cell at the right expresses no or undetectable levels of wt-GFP and shows nonclustered ER (arrows). (B) Another example of an ER cluster in a cell with high expression of wt-GFP. N, nucleus; P, plasma membrane. Bars: (A) 1 μm; (B) 500 nm.