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. 2001 Jun 11;153(6):1287–1300. doi: 10.1083/jcb.153.6.1287

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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FRAP shows fast diffusion for luminal deletion mutants. Quantitative FRAP experiments with transiently transfected COS cells expressing CLIMP-63–GFP constructs. (A) The recovery profiles of five constructs are shown as the mean value of intensity in the region of interest as a function of time. (B) GFP-Δcytoplasmic (left) and GFP-Δcytoplasmic-Δ246–492 (right) show differences in diffusion comparing mobile, reticular ER (○) with immobile, clustered ER (•).