Table 3.
AUC, CD Spectroscopy Data and Melting Curves
| Condition | ||||||
|---|---|---|---|---|---|---|
| Fragments | Quantity of urea | pH level | [Θ]222 | Tm | Sedimentation coefficient s20, w | Molecular mass |
| M | 10−3 deg.cm2/dmol | °C | S | kD | ||
| 127–601 | 1 | 8.0 | −32.6 | 45 | 25.7 | 2547 (55) |
| 127–601 | 1.5 | 8.0 | −20.3 | ND | 80% 26:20% 2 | ND |
| 127–601 | 2 | 8.0 | −13.7 | ND | 50% 26:50% 2 | ND |
| 127–601 | 2.5 | 8.0 | −5.8 | ND | 20% 26:80% 2 | ND |
| 127–601 | 3 | 8.0 | −4.5 | ND | 2.1 | 58 (55) |
| 127–601 | 1 | 6.0 | −33.2 | 45 | 177.0 | ND |
| 127–459 | 1 | 8.0 | −31.6 | 45 | >20 | ND |
| 127–268 | 1 | 8.0 | −14.6 | 45 | >20 | ND |
| 268–459 | 1 | 8.0 | ND | ND | ND | 40 (21) |
Mean molar ellipcities at 222 nm, thermal melting temperatures at the midpoint of transition, sedimentation coefficients, and molecular masses of various CLIMP-63 constructs at different conditions.